polyclonal rabbit anti human egfr igg  (Santa Cruz Biotechnology)

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: ( a ) Western blot analysis of EGFR, Nectin-4, and TROP-2 expression in the BC cell lines RT4 and RT112 and in the antigen-negative control cell line CHO. ( b ) Cell binding of the antibodies and conjugates to the target cells as demonstrated by flow cytometry. Binding of the isotype control antibody is shown in gray.

Article Snippet: Following transfer, EGFR, Nectin-4 and TROP-2 were detected using polyclonal rabbit anti-human EGFR IgG (#sc-03-G, Santa Cruz Biotechnology, Dallas, TX, USA), polyclonal rabbit anti-human Nectin-4 IgG (#17402S, Cell Signaling Technologies, Danvers, MA, USA), and monoclonal rabbit anti-human TROP-2 IgG (#90540, Cell Signaling Technologies).

Techniques: Western Blot, Expressing, Negative Control, Binding Assay, Flow Cytometry, Control

Journal: Molecules

Article Title: Exploring EGFR, Nectin-4, and TROP-2 as Therapeutic Targets for Bladder Cancer Photoimmunotherapy

doi: 10.3390/molecules30244802

Figure Lengend Snippet: Graphical abstract illustrating the future clinical application of PIT using WB692-CB2 conjugates for the treatment of NMIBC. Following intravesical application of the antibody–dye conjugates into the bladder, they can be activated by cystoscopic red light for the selective eradication of BC cells expressing EGFR, Nectin-4 and/or TROP-2. For personalized PIT, a pre-therapeutic determination of target antigen expression in biopsy samples may guide the selection of an optimal conjugate combination. Created in BioRender. Wolf, I (2025) https://BioRender.com/sx866ld .

Article Snippet: Following transfer, EGFR, Nectin-4 and TROP-2 were detected using polyclonal rabbit anti-human EGFR IgG (#sc-03-G, Santa Cruz Biotechnology, Dallas, TX, USA), polyclonal rabbit anti-human Nectin-4 IgG (#17402S, Cell Signaling Technologies, Danvers, MA, USA), and monoclonal rabbit anti-human TROP-2 IgG (#90540, Cell Signaling Technologies).

Techniques: Expressing, Selection

Journal: Bioscience Reports

Article Title: The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer

doi: 10.1042/bsr20181556

Figure Lengend Snippet: Figure 6. CDK6 and EGFR are targets of miR-1296-5p (A) Schematic graph of wild-type and mutant putative binding sites of miR-1296-5p targeting the 3′-UTR of CDK6 and EGFR. (B) Relative luciferase activity of SGC-7901 and GSC-803 cells after cotransfection with wild-type or mutant CDK6 and EGFR 3′-UTR

Article Snippet: Antigen retrieval was achieved by boiling the specimens in a citrate buffer at 120◦C for 3 min. After blocking endogenous peroxides and proteins, the specimens were incubated with anti-human EGFR rabbit polyclonal IgG (1:200; Proteintech; 10515-1-AP) and anti-human CDK6 rabbit polyclonal IgG (1:50; Proteintech; 15508-1-AP) antibodies overnight.

Techniques: Mutagenesis, Binding Assay, Luciferase, Activity Assay, Cotransfection

Journal: Bioscience Reports

Article Title: The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer

doi: 10.1042/bsr20181556

Figure Lengend Snippet: Figure 7. EGFR and CDK6 are down-regulated in GC and negatively correlated with miR-1296-5p

Article Snippet: Antigen retrieval was achieved by boiling the specimens in a citrate buffer at 120◦C for 3 min. After blocking endogenous peroxides and proteins, the specimens were incubated with anti-human EGFR rabbit polyclonal IgG (1:200; Proteintech; 10515-1-AP) and anti-human CDK6 rabbit polyclonal IgG (1:50; Proteintech; 15508-1-AP) antibodies overnight.

Techniques:

Journal: Bioscience Reports

Article Title: The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer

doi: 10.1042/bsr20181556

Figure Lengend Snippet: Figure 8. Downregulation of EGFR and CDK6 are key to the antiproliferative function of miR-1296-5p (A) Representative Western blots for CDK6 and EGFR in SGC-7901 and GSC-803 cells. (B) Cell viability was examined by an MTT assay in SGC-7901 and GSC-803 cells. Bar graphs represent the mean +−SEM from at least three independent experiments;

Article Snippet: Antigen retrieval was achieved by boiling the specimens in a citrate buffer at 120◦C for 3 min. After blocking endogenous peroxides and proteins, the specimens were incubated with anti-human EGFR rabbit polyclonal IgG (1:200; Proteintech; 10515-1-AP) and anti-human CDK6 rabbit polyclonal IgG (1:50; Proteintech; 15508-1-AP) antibodies overnight.

Techniques: Western Blot, MTT Assay

Journal: Bioscience Reports

Article Title: The tumor-suppressive function of miR-1296-5p by targeting EGFR and CDK6 in gastric cancer

doi: 10.1042/bsr20181556

Figure Lengend Snippet: Figure 9. Overexpression of EGFR and CDK6 reversed the inhibitory effect of miR-1296-5p on cell invasion Representative images of invasive cells on polycarbonate Transwell membranes. Bar graphs represent the average number of invasive cells per field from three independent experiments; *P<0.05, **P<0.01, and ***P<0.001 vs. the indicated groups.

Article Snippet: Antigen retrieval was achieved by boiling the specimens in a citrate buffer at 120◦C for 3 min. After blocking endogenous peroxides and proteins, the specimens were incubated with anti-human EGFR rabbit polyclonal IgG (1:200; Proteintech; 10515-1-AP) and anti-human CDK6 rabbit polyclonal IgG (1:50; Proteintech; 15508-1-AP) antibodies overnight.

Techniques: Over Expression